Does Aphid Feeding Trigger the Production of H202 in Wild Barley?

Authors

Richard Justin Holewa, Augsburg University

Files

Faculty Mentor

Leon van Eck

Description

In an in vivo experiment to see if the oxidative burst of reactive oxygen species (ROS) is utilized as a defense mechanism in barley against the bird cherry-oat aphid (Rhopalosiphum padi), aphids were pre- starved then allowed to feed on cultivated (Hordeum vulgare) and wild barley (H. spontaneum) accessions. Hydrogen peroxide (H2O2) generated in barley leaves in response to aphid feeding was quantified with DAB (3,3'-diaminobenzidine) staining. Stained leaves were digitized and analyzed using Fiji. Proportions of stained areas were statistically tested for significance (with pooled t-tests and ANOVA at 5%). No significant differences were detected between wild and cultivated barley, nor between aphid-challenged and control tissues. Therefore, ROS are not a notable component of barley’s defense machinery when preyed upon by R. padi.

Publication Date

2022

Keywords

aphids, barley

Disciplines

Biology

Recommended Citation

Holewa, Richard Justin, "Does Aphid Feeding Trigger the Production of H202 in Wild Barley?" (2022). All Zyzzogeton Presentations. 27.
https://idun.augsburg.edu/zyzzogeton/27