Cobalt Release from a Nanoscale Multiphase Lithiated Cobalt Phosphate Dominates Interaction with Shewanella oneidensis MR-1 and Bacillus subtilis SB491

Authors

Peter L. Clement, University of Minnesota - Twin Cities
Joshua E. Kuether, Augsburg UniversityFollow
Jaya R. Borgatta, University of Wisconsin-Madison
Joseph T. Buchman, University of Minnesota - Twin Cities
Meghan S. Cahill, University of Minnesota - Twin Cities
Tian A. Qiu, University of Minnesota - Twin Cities
Robert J. Hamers, University of Wisconsin-Madison
Z Vivian Feng, Augsburg UniversityFollow
Christy L. Haynes, University of Minnesota - Twin Cities

ORCID

0000-0002-3329-3781

Document Type

Article

Publication Date

2-4-2020

DOI

10.1021/acs.chemrestox.9b00465

Abstract

Cobalt phosphate engineered nanomaterials (ENMs) are an important class of materials that are used as lithium ion battery cathodes, catalysts, and potentially as super capacitors. As production of these nanomaterials increases, so does the likelihood of their environmental release; however, to date, there are relatively few investigations of the impact of nanoscale metal phosphates on biological systems. Furthermore, nanomaterials used in commercial applications are often multiphase materials, and analysis of the toxic potential of mixtures of nanomaterials has been rare. In this work, we studied the interactions of two model environmental bacteria, Shewanella oneidensis MR-1 and Bacillus subtilis, with a multiphase lithiated cobalt phosphate (mLCP) nanomaterial. Using a growth-based viability assay, we found that mLCP was toxic to both bacteria used in this study. To understand the observed toxicity, we screened for production of reactive oxygen species (ROS) and release of Co²⁺ from mLCP using three abiotic fluorophores. We also used Newport Green DCF dye to show that cobalt was taken up by the bacteria after mLCP exposure. Using transmission electron microscopy, we noted that the mLCP was not associated with the bacterial cell surface. In order for us to further probe the mechanism of interaction of mLCP, the bacteria were exposed to an equivalent dose of cobalt ions that dissolved from mLCP, which recapitulated the changes in viability when the bacteria were exposed to mLCP, and it also recapitulated the observed bacterial uptake of cobalt. Taken together, this implicates the release of cobalt ions and their subsequent uptake by the bacteria as the major toxicity mechanism of mLCP. The properties of the ENM govern the release rate of cobalt, but the toxicity does not arise from nanospecific effects—and importantly, the chemical composition of the ENM may dictate the oxidation state of the metal centers and thus limit ROS production.

Comments

Final version published in Chemical Research in Toxicology at https://pubs.acs.org/doi/abs/10.1021/acs.chemrestox.9b00465

Recommended Citation

Clement, P.L; Kuether, J.E.; Borgatta, J.R.; Buchman, J.T.; Cahill, M.S.; Qiu, T.A.; Hamers, R.J.; Feng, Z.V.; Haynes, C.L., “Cobalt Release from a Nanoscale Multiphase Lithiated Cobalt Phosphate Dominates Interaction with Shewanella oneidensis MR-1 and Bacillus subtilis SB491”, Chem. Res. in Toxicol., 2020, 33 (3), 806-816.