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Matthew Backman, PhD
Manganese (Mn) is an essential trace metal required for the normal physiological processes in humans. It plays an important role as a constituent of multiple enzymes and an activator of other enzymes. However, elevated cellular levels are toxic and may lead to the development of an irreversible Parkinson like syndrome that has no treatment. Recently, a new form of homozygous mutations in SLC30A10 causes familial parkinsonism associated with manganese (Mn) retention. SLC30A10 is a cell surface-localized Mn efflux transporter that reduces cellular Mn levels and protects against Mn-induced toxicity. The discoveries of these genetic diseases have transformed our understanding of Mn homeostasis, detoxification, and neurotoxicity. Gene expression analysis is increasingly vital in many fields of biological research. Validation of the real-time reverse transcription polymerase chain reaction (RT-qPCR) technique is a very accurate and sensitive method and becoming widely used to quantify gene expression from neuroblastoma cells. It can be used to quantify mRNA expression levels. This method allows for the direct detection of PCR products combining amplification and detection in one single step.
Pharmacology, Toxicology and Environmental Health
Darkazanli, Yusra, "Development of RT-qPCR Assay for a Mn Transport Gene Expression in Human Neuroblastoma Cells" (2020). All Zyzzogeton Presentations. 22.